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| * '''Description:''' [[two-component systems|two-component]] system response regulator, controls gene expression in response to cell density (concentration of [[ComX]]) <br/><br/>
| | {{#bank: ComA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''comA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''srfB, comAA ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] system response regulator
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of [[genetic competence]] and [[quorum sensing]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31680 comA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ComA ComA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 4.759
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 642 bp, 214 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxO]]'', ''[[comP]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31680 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:comA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comA_3252804_3253448_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:comA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31680]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[genetic competence]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[quorum sensing]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The [[ComA regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU31680
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31680&redirect=T BSU31680]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comA-yuxO.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10381]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' ComA family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:''' phosphorylated by [[ComP]] on an Asp residue
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** phosphorylation activates DNA binding
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| ** DNA-binding activity of ComA is inhibited by [[RapC]] {{PubMed|12950917}}, [[RapD]] {{PubMed|17227471}}, [[RapF]] {{PubMed|15968044,22215984}}, [[RapH]] {{PubMed|17581123}} and [[RapK]] {{PubMed|16816200}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[RapC]]-[[ComA]] {{PubMed|12950917}}
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| ** [[RapF]]-[[ComA]] {{PubMed|15968044,22215984}}
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| ** [[RapH]]-[[ComA]] {{PubMed|17581123}}
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| ** [[ComP]]-[[ComA]]
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31680&redirect=T BSU31680]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=1U83 1U83]
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=3ULQ 3ULQ] (structure of the complex between the DNA-binding C-terminal domain of [[ComA]] with [[RapF]]) {{PubMed|22215984}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P14204 P14204]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31680]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.4.1.19 4.4.1.19]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[comA]]-[[yuxO]]'' {{PubMed|2507523}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comA_3252804_3253448_-1 comA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2507523}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 320 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 623 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>20030732 12576575 </pubmed>
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| ==Original Publications==
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| <pubmed>17227471 11133937,2116363,12067344,8387999,19202088, 10094672,8643670,11267663, 15968044 16816200, 10447896,12950917, 20302877 12270822 17581123 16816200 2507523 20208433 22215984 24130811 24425772 24788106 25598361</pubmed>
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| [[Category:Protein-coding genes]]
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