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| * '''Description:''' glutamate 5-kinase <br/><br/>
| | {{#bank: ProJ}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''proJ''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yohA ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || glutamate 5-kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || osmoadaptive de novo production of proline
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18470 proJ]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=proJ proJ]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.296
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1113 bp, 371 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gltC]]'', ''[[proH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18470 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:proJ_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proJ_2015733_2016848_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:proJ_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18470]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
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| {{SubtiWiki category|[[coping with hyper-osmotic stress]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU18470
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18470&redirect=T BSU18470]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12658]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[ProB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18470&redirect=T BSU18470]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O07509 O07509]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18470]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.2.11 2.7.2.11]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[proH]]-[[proJ]]'' {{PubMed|21296969}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proJ_2015733_2016848_-1 proJ] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|21784929}}
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| * '''Regulation:'''
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| ** expressed under conditions of osmotic stress {{PubMed|21784929}}
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| ** induced by cold stress {{PubMed|21296969}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP817 (spc) available in the [[Stülke]] lab
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| ** 1A635 ( ''proJ''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>,21296969 25344233 21784929</pubmed>
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| [[Category:Protein-coding genes]]
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