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| * '''Description:''' cell-division inhibitor (septum placement), destabilizes [[FtsZ]]-rings at new cell poles, part of the Min system (with [[DivIVA]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement <br/><br/>
| | {{#bank: MinC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''minC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || cell-division inhibitor
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| |style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28000 minC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MinC MinC]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 6.262
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 678 bp, 226 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[minD]]'', ''[[mreD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28000 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:minC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minC_2858584_2859264_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:minC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28000]]
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| |-
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| |}
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|
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell division]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigH regulon]]}},
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| {{SubtiWiki regulon|[[SigM regulon]]}}
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|
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| =The gene=
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|
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| === Basic information ===
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| * '''Locus tag:''' BSU28000
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|
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| ===Phenotypes of a mutant ===
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| * a ''[[yvcL]] [[minC]]-[[minD]]'' mutant grows poorly, and the cells are filamentous {{PubMed|24097947}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28000&redirect=T BSU28000]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10329]
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|
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| === Additional information===
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|
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| =The protein=
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|
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle {{PubMed|20352045}}
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| ** binds the C-terminal domain of [[FtsZ]] to inhibit its polymerization {{PubMed|23577149}}
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| * '''Protein family:''' minC family (according to Swiss-Prot)
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|
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| * '''Paralogous protein(s):'''
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}
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| ** [[FtsZ]] (C-terminal domain)-[[MinC]] {{PubMed|23577149}}
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|
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| * '''[[Localization]]:'''
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| ** polar/ septal at the cell membrane {{PubMed|20566861}}
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| ** membrane binding/ polar localization depends on the proton motive force {{PubMed|20566861}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28000&redirect=T BSU28000]
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|
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| * '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=2M4I 2M4I] (N-terminal domain, 105 aa)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q01463 Q01463]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28000]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[radC]]-[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
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| ** ''[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]'' {{PubMed|8459776}}
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| ** ''[[minC]]-[[minD]]'' {{PubMed|8459776}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minC_2858584_2859264_-1 minC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[radC]]'': [[SigM]] {{PubMed|18179421}}
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| ** ''[[minC]]'': [[SigH]] {{PubMed|8459776}}
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| * '''Regulation:'''
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| ** expression is reduced in a [[SigV]] mutant {{PubMed|21926231}}
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| ** constitutively expressed {{PubMed|23701187}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 95 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' BSN375 (''[[minC]]''::''aphA3'') (available in [[Leendert Hamoen]]'s, [[Sven Halbedel]]'s and [[Jörg Stülke]]'s labs)
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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|
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 19680248 19884039 24367361</pubmed>
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| ==Original Publications==
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| <pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 24097947 18179421 22457634 23577149 21926231 23701187 24366721</pubmed>
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| [[Category:Protein-coding genes]]
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