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| * '''Description:''' guanylate kinase (GMP:dATP, dGMP:ATP) <br/><br/>
| | {{#bank: Gmk}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gmk''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yloD ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || guanylate kinase (GMP:dATP, dGMP:ATP)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || GTP biosynthesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15680 gmk]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gmk gmk]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 4.616
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 612 bp, 204 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylzA]]'', ''[[yloH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15680 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:gmk_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gmk_1641949_1642563_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gmk_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15680]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU15680
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15680&redirect=T BSU15680]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13386]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + GMP = ADP + GDP (according to Swiss-Prot)
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| * '''Protein family:''' guanylate kinase-like domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| ** phosphorylated on Arg-134 and/or Arg-149{{PubMed|22517742}}
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´{{PubMed|22981860}}
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15680&redirect=T BSU15680]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1S4Q 1S4Q] (from ''Mycobacterium tuberculosis h37rv'', 39% identity, 60% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34328 O34328]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15680]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.4.8 2.7.4.8]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gmk_1641949_1642563_1 gmk] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 254 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1208 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1529 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 895 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1794 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>22517742 22981860 24722991 24682323 25661490</pubmed>
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| [[Category:Protein-coding genes]]
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