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| * '''Description:''' valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase <br/><br/>
| | {{#bank: Bcd}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''bcd''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''bkd, yqiT ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || valine dehydrogenase, isoleucine dehydrogenase, <br/>L-leucine dehydrogenase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24080 bcd]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=bcd bcd]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 4.942
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1092 bp, 364 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[buk]]'', ''[[ptb]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24080 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24080 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24080 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:bcd_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bcd_2502659_2503753_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:bcd_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24080]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of amino acids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[BkdR regulon]]}},
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| {{SubtiWiki regulon|[[SigL regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24080
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24080&redirect=T BSU24080]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11723]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' L-leucine + H<sub>2</sub>O + NAD<sup>+</sup> = 4-methyl-2-oxopentanoate + NH<sub>3</sub> + NADH (according to Swiss-Prot)
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| * '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24080&redirect=T BSU24080]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1LEH 1LEH] (from '' Lysinibacillus sphaericus'', 71% identity, 82% similarity) {{PubMed|8591046}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54531 P54531]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24080]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.9 1.4.1.9]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bcd_2502659_2503753_-1 bcd] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
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| * '''Regulation:'''
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| ** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}
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| * '''Regulatory mechanism:'''
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| ** [[CodY]]: indirect regulation {{PubMed|24843172}}
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| ** [[BkdR]]: transcription activation {{PubMed|10094682}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3408 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2690 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 11556 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** a ''bcd'' mutant and a ''[[bcd]] [[ybgE]] [[ywaA]]'' triple mutant are available in [[Linc Sonenshein]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>7705101</pubmed>
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| ==Original publications==
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| <pubmed>12427936,15241682,10094682,12823818, 24843172</pubmed>
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| [[Category:Protein-coding genes]]
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