AddA: Difference between revisions
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=== Additional information=== | === Additional information=== | ||
* A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}} | * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}} | ||
=The protein= | =The protein= | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=addA_1139807_1143505_1 addA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=addA_1139807_1143505_1 addA] {{PubMed|22383849}} | ||
* '''Sigma factor:''' [[SigA]] {{PubMed|7746142}} | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7746142}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
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=Biological materials = | =Biological materials = | ||
* '''Mutant:''' GP1106 ( | * '''Mutant:''' GP1106 ([[addA]]-[[addB]], spc), available in [[Jörg Stülke]]'s lab | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
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==Original publications== | ==Original publications== | ||
<pubmed>,8387145,15610857,7746142, 19129187 15009890 10756102 9781875 16632468 17570399 20350930 21809208 21821766 22307084 24682829 24670664 22383849 21071401 8752329 8752323 </pubmed> | <pubmed>,8387145,15610857,7746142, 19129187 15009890 10756102 9781875 16632468 17570399 20350930 21809208 21821766 22307084 24682829 24670664 22383849 21071401 8752329 8752323 25486468 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:28, 11 December 2014
- Description: ATP-dependent deoxyribonuclease (subunit A)
| Gene name | addA |
| Synonyms | recE5 |
| Essential | no |
| Product | ATP-dependent deoxyribonuclease (subunit A)) |
| Function | DNA repair/ recombination |
| Gene expression levels in SubtiExpress: addA | |
| Interactions involving this protein in SubtInteract: AddA | |
| MW, pI | 140 kDa, 5.127 |
| Gene length, protein length | 3696 bp, 1232 aa |
| Immediate neighbours | addB, sbcD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Categories containing this gene/protein
DNA repair/ recombination, genetic competence
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10630
Phenotypes of a mutant
Database entries
- BsubCyc: BSU10630
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- the enzyme is functional as a heterodimer of the AddA and AddB subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'→5' polarity located in the AddA subunit PubMed
- the AddB subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences PubMed
- Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU10630
- UniProt: P23478
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 147 PubMed
Biological materials
- Mutant: GP1106 (addA-addB, spc), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Mark Dillingham, Bristol, U.K. (homepage)
Your additional remarks
References
Reviews
<pubmed> 23202527, 20116346 22933559 19542287 </pubmed>
Original publications
<pubmed>,8387145,15610857,7746142, 19129187 15009890 10756102 9781875 16632468 17570399 20350930 21809208 21821766 22307084 24682829 24670664 22383849 21071401 8752329 8752323 25486468 </pubmed>