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| * '''Description:''' [[trigger enzymes|trigger enzyme]]: aconitase and RNA binding protein<br/><br/>
| | {{#bank: CitB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''citB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: aconitate hydratase (aconitase)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18000 citB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CitB CitB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=citB citB]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 99 kDa, 4.903
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2727 bp, 909 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sspO]], [[yneN]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18000 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:citB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=citB_1926680_1929409_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:citB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18000]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[trigger enzyme]]}},
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| {{SubtiWiki category|[[RNA binding regulators]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[CcpC regulon]]}},
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[FsrA regulon]]}}
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|
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| =The [[CitB regulon]]: ''[[feuA]]-[[feuB]]-[[feuC]]-[[ybbA]]'', ''[[citZ]]''=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU18000
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| ===Phenotypes of a mutant ===
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| * glutamate auxotrophy and a defect in sporulation [http://www.ncbi.nlm.nih.gov/pubmed/9393699 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18000&redirect=T BSU18000]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/citB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10478]
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| === Additional information===
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| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** Citrate <=> isocitrate
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| ** Binding to iron responsive elements (IRE RNA) in the absence of the FeS cluster {{PubMed|23354745,10468622}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:''' FeS cluster
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[Mdh]]-[[CitB]] {{PubMed|20933603}}
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| ** [[CitG]]-[[CitB]] {{PubMed|20933603}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18000&redirect=T BSU18000]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1L5J 1L5J] (''E. coli'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P09339 P09339]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18000]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.3 4.2.1.3]
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| === Additional information===
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| * ''B. subtilis'' aconitase is both an enzyme and an RNA binding protein ([[moonlighting proteins|moonlighting protein]]) [http://www.ncbi.nlm.nih.gov/pubmed/10468622 PubMed]
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| * extensive information on the structure and enzymatic properties of CitB can be found at [http://www.proteopedia.org/wiki/index.php/Aconitase Proteopedia]
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| =Expression and regulation=
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| * '''Operon:''' ''[[citB]]'' (according to [http://dbtbs.hgc.jp/COG/prom/citB.html DBTBS])
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=citB_1926680_1929409_1 citB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1310745}}
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| * '''Regulation:'''
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12591885 PubMed]
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| ** repressed in the presence of glucose and glutamate ([[CcpC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10656796 PubMed]
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| ** expressed upon transition into the stationary phase ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12591885 PubMed], indirect negative regulation by [[AbrB]] {{PubMed|20817675}}
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| ** repressed by glucose (3.7-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
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| ** repression by glucose + arginine ([[CcpC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16395550 PubMed]
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| ** less expressed under conditions of extreme iron limitation ([[FsrA]]) [http://www.ncbi.nlm.nih.gov/pubmed/18697947 PubMed]
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| ** part of the iron sparing response ([[FsrA]]) {{PubMed|22389480}}
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| * '''Regulatory mechanism:'''
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12591885 PubMed]
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| ** [[CcpC]]: transcription repression (molecular inducer: citrate) [http://www.ncbi.nlm.nih.gov/sites/entrez/10656796 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12100558 PubMed2]
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| ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12100558 PubMed]
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| ** [[FsrA]]: translational repression {{PubMed|18697947}}
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| * '''Additional information:'''
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 5049 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 24371 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5745 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3428 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5655 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP1275 (''citB''::''erm''), available in [[Jörg Stülke]]'s lab
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| ** GP1441 (spc), available in [[Jörg Stülke]]'s lab
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| ** 1A999 ( ''citB''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]
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| * '''Expression vector:
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| **GP1439 (''citB''-''Strep'' ''(spc)''), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab
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| **pGP1810 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| ** pGP700 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab
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| * '''GFP fusion:''' GP1434 (spc, based on [[pGP1870]]), available in [[Jörg Stülke]]'s lab
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
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| * '''Antibody:''' available in [[Linc Sonenshein]]'s lab
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| * '''FLAG-tag construct:'''
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| ** GP1144 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
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| ** GP1145 (kan), available in [[Jörg Stülke]]'s lab
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| =Labs working on this gene/protein=
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| * [[Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
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| * [[Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 12732309 2696478 18261896 18086213 </pubmed>
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| ==Original publications==
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| <pubmed>18697947, 20097860,2118511,12591885,10656796,12591885,12850135 2413006 10656796 10468622 6143742 12591885 16395550 16923907 9642180 9393699 12591885 2105305,20933603 21099137 21446632 21821766 22389480 23139400 23354745 20817675 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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