Methods: Difference between revisions
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* Visualization and quantification of gene expression heterogeneity in growing microbial cells {{PubMed|23874729}} | * Visualization and quantification of gene expression heterogeneity in growing microbial cells {{PubMed|23874729}} | ||
* GFP variants specifically optimizd for use in ''B. subtilis'' {{PubMed|23956387}} | * GFP variants specifically optimizd for use in ''B. subtilis'' {{PubMed|23956387}} | ||
* Quantitative analysis of cell types by comparing microscoy images {{PubMed|25448819}} | |||
==Transposon mutagenesis== | ==Transposon mutagenesis== | ||
Revision as of 17:56, 5 December 2014
Here you can find a list of methods for working with Bacillus subtilis:
Media
Analysis of protein-protein interactions
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- in vivo detection of protein-protein interactions using DivIVA and GFP PubMed
Analysis of membrane proteins
- application of a split green fluorescent protein reporter PubMed
Protein expression
- the LIKE system PubMed
Strain construction
- introduction of markerfree deletions: PubMed
- generation of markerfree mutations: PubMed
- an improved method for transformation: PubMed
RNA analysis
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
Genetic work with non-standard strains
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
- efficient electroporation of B. subtilis PubMed
- a novel transformation protocol for B. subtilis DB104 PubMed
General procedures
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
Microscopic techniques
- Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
- Assay of gene expression dynamics using live cell imaging PubMed
- Visualization and quantification of gene expression heterogeneity in growing microbial cells PubMed
- GFP variants specifically optimizd for use in B. subtilis PubMed
- Quantitative analysis of cell types by comparing microscoy images PubMed
Transposon mutagenesis
- mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed
Protein-DNA interactions
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
Genome engineering
- generalized bacterial genome editing using mobile group II introns and Cre-lox PubMed
- A review on genome engineering:
<pubmed>23340847 </pubmed>
Visual descriptions of methods for B. subtilis in the Journal of Visual Experimentation
<pubmed> 24473333 24457605 24300024 24300445 22371091 20142799 21841760 </pubmed>
Key references
<pubmed>22179594 20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 23956387 22285978,22113911 24002656 24282588 </pubmed>