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| * '''Description:''' elongation factor G, facilitates movement of tRNA–mRNA by one codon <br/><br/>
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''fusA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''fus ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || elongation factor G
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01120 fusA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FusA FusA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 76 kDa, 4.615
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2076 bp, 692 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpsG]]'', ''[[tufA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01120 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01120 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01120 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:fusA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fusA_130684_132762_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:fusA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01120]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU01120
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01120&redirect=T BSU01120]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11939]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' hydrolyses GTP
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| * '''Protein family:''' EF-G/EF-2 subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:''' phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[FusA]]-[[RplL]] {{PubMed|23912278}}
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| ** [[FusA]]-[[RpsL]] {{PubMed|23912278}}
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01120&redirect=T BSU01120]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1ELO 1ELO] (from ''Thermus thermophilus'') {{PubMed|8070397}}
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| ** [http://www.pdb.org/pdb/explore/explore.do?structureId=4BTC 4BTC], [http://www.pdb.org/pdb/explore/explore.do?structureId=4BTD 4BTD], the ''Thermus thermophilus'' EF-G–[[ribosome]] complex in a pretranslocation state {{PubMed|23912278}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P80868 P80868]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01120]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fusA_130684_132762_1 fusA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 11452 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 49524 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 23259 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 12858 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 16612 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP840, available in [[Jörg Stülke]]'s lab
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| ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP848, available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed> 24614227 17726680, 17218307 20348921 8070397 23912278 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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