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| * '''Description:''' component of the SpoIIIA-[[SpoIIQ]] type III secretion system residing in the forespore membrane, required for [[SigG]] activation<br/><br/>
| | {{#bank: SpoIIIAH}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spoIIIAH''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || part of the transmembrane channel linking the mother cell and the forespore
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || activation of [[SigG]], forespore encasement by the spore coat
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24360 spoIIIAH]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SpoIIIAH SpoIIIAH]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 4.627
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 654 bp, 218 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[accB]]'', ''[[spoIIIAG]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24360 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spoIIIAH_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIIAH_2532353_2533009_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spoIIIAH_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24360]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein secretion]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigE regulon]]}},
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| {{SubtiWiki regulon|[[SpoIIID regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24360
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| ===Phenotypes of a mutant ===
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| * block of sporulation after engulfment
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24360&redirect=T BSU24360]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqhV-spoIIIAABCDEFGH.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11415]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** required for forespore encasement by the spore coat {{PubMed|22171814}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SpoIIIAH]]-[[SpoIIQ]] (interaction between proteins membrane proteins expressed in the mother cell and the forespore, respectively; the complex forms a 60 Å channel made up of twelve subunits of each protein) {{PubMed|22431604,22431613,15574594,21097616}}
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| * '''[[Localization]]:'''
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| ** membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with [[SpoIIQ]]) {{PubMed|22431604,22431613,22171814,18485064}}
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| ** proper recruitment to the sporulation septum on the mother cell side requires [[SpoIIQ]] {{PubMed|23834622}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24360&redirect=T BSU24360]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=3UZ0 3UZO]; [http://www.rcsb.org/pdb/explore/explore.do?structureId=3TUF 3TUF] (the [[SpoIIQ]]-[[SpoIIIAH]] pore forming complex) {{PubMed|22431604,22431613}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P49785 P49785]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24360]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[spoIIIAA]]-[[spoIIIAB]]-[[spoIIIAC]]-[[spoIIIAD]]-[[spoIIIAE]]-[[spoIIIAF]]-[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
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| ** ''[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIIAH_2532353_2533009_-1 spoIIIAH] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190}}
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| ** both promoters: [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
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| * '''Regulation:'''
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| ** both promoters: expressed during sporulation in the mother cell ([[SigE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed], switched off by [[SpoIIID]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
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| * '''Regulatory mechanism:''' [[SpoIIID]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
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| * '''Additional information:''' the internal promoter is essential for sporulation, it is twice as active as the promoter in front of [[spoIIIAA]], suggesting that [[SpoIIIAG]] and [[SpoIIIAH]] are required in larger amounts as compared to the other products of the operon [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 23944268 25105965 </pubmed>
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| == Original publications ==
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| <pubmed>15752199,18485064,15574594,18812514,1766372,17693505,19609349 , 17121846 21097616 22431613 22171814 23834622 22431604 23859254 </pubmed>
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| [[Category:Protein-coding genes]]
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