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| * '''Description:''' [[two-component systems|two-component]] sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with [[cell division]] by controlling the transcription of genes for autolysins and their inhibitors <br/><br/>
| | {{#bank: WalK}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''walK''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yycG''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] sensor kinase (OmpR family)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of cell wall metabolism
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40400 walK]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=WalK WalK]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 69 kDa, 4.775
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1833 bp, 611 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yycH]]'', ''[[walR]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40400 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40400 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40400 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yycG_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walK_4151853_4153688_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:walK_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40400]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell wall/ other]]}},
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU40400
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40400&redirect=T BSU40400]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yycFGHIJ-yyxA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11461]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of [[WalR]]
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| * '''Protein family:''' two-component sensor kinase of the OmpR family
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
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| * '''Modification:''' autophosphorylation on a His residue
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[WalK]]-[[WalR]]
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| ** [[WalK]]-[[YycH]] {{PubMed|21219466}},
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| ** [[WalK]]-[[YycI]] {{PubMed|21219466}}
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| ** [[WalK]]-[[DivIB]] {{PubMed|21219466}}
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| ** [[WalK]]-[[FtsL]] {{PubMed|21219466}}
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| ** [[WalK]]-[[PbpB]] {{PubMed|21219466}}
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| ** [[WalK]]-[[FtsZ]] {{PubMed|18573169}}
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| * '''[[Localization]]:'''
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| ** cell membrane {{PubMed|21219466}}
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| ** part of the division septum in growing cells, interacts with the [[divisome]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18573169 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40400&redirect=T BSU40400]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=3sl2 3SL2] (complex of the ATP binding histidine kinase domain with ATP) {{PubMed|22751669}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q45614 Q45614]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40400]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[walR]]-[[walK]]-[[yycH]]-[[yycI]]-[[walJ]]-[[htrC]]'' {{PubMed|9829949}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walK_4151853_4153688_-1 walK] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' expressed during vegetative growth, repressed during stationary phase [http://www.ncbi.nlm.nih.gov/sites/entrez/9829949 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 236 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| ** pGP2636, constitutive expression in ''B. subtilis'' (based on [[pBQ200]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19019149,18792692,10094672, 18245295 </pubmed>
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| ==Original Publications==
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| <pubmed>18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157, 19342776 20057515 20059685 20167622 21219466 23632918 22751669,22526318 24490131 24900578 </pubmed>
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| [[Category:Protein-coding genes]]
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