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| * '''Description:''' [[ribosomal protein]] <br/><br/>
| | {{#bank: RplF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rplF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|23002217,12682299}}
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[ribosomal protein]] L6 (BL8)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01310 rplF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RplF RplF]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 9.916
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 537 bp, 179 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpsH]]'', ''[[rplR]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01310 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01310 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01310 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rplF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rplF_142402_142941_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rplF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01310]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| {{SubtiWiki category|[[universally conserved proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU01310
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| ===Phenotypes of a mutant ===
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| essential {{PubMed|23002217,12682299}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01310&redirect=T BSU01310]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11408]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' [[ribosomal protein]] L6P family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[YsxC]]-[[RplF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17981968 PubMed]
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01310&redirect=T BSU01310]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1RL6 1RL6] (Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=2R6A 2R6A] (Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=2R6C 2R6C], [http://www.rcsb.org/pdb/explore.do?structureId=2R6D 2R6D], [http://www.rcsb.org/pdb/explore.do?structureId=2R6E 2R6E]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P46898 P46898]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01310]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[rpsJ]]-[[rplC]]-[[rplD]]-[[rplW]]-[[rplB]]-[[rpsS]]-[[rplV]]-[[rpsC]]-[[rplP]]-[[rpmC]]-[[rpsQ]]-[[rplN]]-[[rplX]]-[[rplE]]-[[rpsN]]-[[rpsH]]-[[rplF]]-[[rplR]]-[[rpsE]]-[[rpmD]]-[[rplO]]'' {{PubMed|9371452}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rplF_142402_142941_1 rplF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** [[SigA]] two promoters, about 140bp and 200bp upstream of the start codon {{PubMed|9371452}}
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| * '''Regulation:'''
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| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 2870 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 8119 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>17981968,8635744,9371452, 19653700 11948165 23002217</pubmed>
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| [[Category:Protein-coding genes]]
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