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| * '''Description:''' DNA mismatch repair <br/><br/>
| | {{#bank: MutL}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mutL''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || DNA mismatch repair
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| |style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17050 mutL]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MutL MutL]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 70 kDa, 5.583
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1881 bp, 627 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mutS]]'', ''[[ymzD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17050 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mutL_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutL_1778337_1780220_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mutL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17050]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU17050
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| ===Phenotypes of a mutant ===
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| * 60-fold increased mutation rate {{PubMed|23882084}}
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| * a ''[[rnhB]] [[mutS]]-[[mutL]]'' mutant is not viable {{PubMed|23882084}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17050&redirect=T BSU17050]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mutSL.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11402]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' DNA mismatch repair mutL/hexB family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[MutL]] (C-terminal domain)-[[DnaN]] {{PubMed|21050827}}
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| ** [[DnaE]]-[[MutL]] {{PubMed|21958350}}
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| ** [[MutL]]-[[MutS]] {{PubMed|23998896}}
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| * '''[[Localization]]:'''
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| ** forms foci at midcell position, the frequency of foci increases upon mismatch formation {{PubMed|21958350}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17050&redirect=T BSU17050]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3KDK 3KDK] (C-terminal domain) {{PubMed|20603082}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P49850 P49850]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17050]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[mutS]]-[[mutL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8760914 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutL_1778337_1780220_1 mutL] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** A [[ncRNA]] is predicted between ''[[mutL]]'' and ''[[ymzD]]'' {{PubMed|20525796}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP1190 (del ''[[mutS]]-[[mutL]]''::''aphA3'') {{PubMed|22178973}}, available in [[Jörg Stülke]]'s lab
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| ** 1A833 ( ''mutL''::''spec''), {{PubMed|11779496}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A833&Search=1A833 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 22933559 </pubmed>
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| == Original publications ==
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| <pubmed>8760914, 15375129, 20603082 20525796 22843852 21050827,21958350 22178973 23882084 23998896 </pubmed>
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| [[Category:Protein-coding genes]]
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