ProH

• Description: pyrroline-5-carboxylate reductase
  
  

Gene name	proH
Synonyms	yoxE
Essential	no
Product	pyrroline-5-carboxylate reductase
Function	osmoadaptive de novo production of proline
Gene expression levels in SubtiExpress: proH
Metabolic function and regulation of this protein in SubtiPathways: proH
MW, pI	31 kDa, 7.754
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	proJ, rtp
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU18480

Database entries

• BsubCyc: BSU18480

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)

• Protein family: glutamate 5-kinase family (according to Swiss-Prot)

• Paralogous protein(s): ProI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU18480

• Structure:

• UniProt: P14383

• KEGG entry: [2]

• E.C. number: 1.5.1.2

Expression and regulation

• Operon: proH-proJ PubMed

• Expression browser: proH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of osmotic stress PubMed
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed> ,11418582, 21296969 25344233 21784929</pubmed>