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| * '''Description:''' valyl-tRNA synthetase <br/><br/>
| | {{#bank: ValS}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''valS''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || valyl-tRNA synthetase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28090 valS]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=valS valS]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 101 kDa, 4.868
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2640 bp, 880 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[folC]]'', ''[[yszA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28090 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:valS_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=valS_2866664_2869306_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:valS_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28090]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[T-box]]}},
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| [[Efp-dependent proteins]]
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU28090
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28090&redirect=T BSU28090]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/valS-folC.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10321]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val) (according to Swiss-Prot)
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| * '''Protein family:''' ValS type 1 subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28090&redirect=T BSU28090]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1IYW 1IYW] (from ''Thermus thermophilus'', 41% identity, 60% similarity) {{PubMed|12554880}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q05873 Q05873]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28090]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/6.1.1.9 6.1.1.9]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[valS]]-[[folC]]'' {{PubMed|9098041}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=valS_2866664_2869306_-1 valS] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9098041}}
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| * '''Regulation:'''
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| ** induced by valine limitation ([[T-box]]) {{PubMed|9098041,19258532}}
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| * '''Regulatory mechanism:'''
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| ** [[T-box]]: [[riboswitch]], transcription termination/antitermination {{PubMed|9098041,19258532}}
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| * '''Additional information:'''
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| ** [[translation]] is likely to require [[Efp]] due to the presence of several consecutive proline residues {{PubMed|23239624,23239623}}
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1169 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 3552 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19258532,, </pubmed>
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| ==Original Publications==
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| <pubmed>,9098041, 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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