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| * '''Description:''' thiol-disulfide oxidoreductase, required for the formation of thiol disulfide bonds in several proteins <br/><br/>
| | {{#bank: BdbD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''bdbD''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvgV ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || thiol-disulfide oxidoreductase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || oxidative folding of proteins
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33480 bdbD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=BdbD BdbD]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.089
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 666 bp, 222 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bdbC]]'', ''[[cadA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33480 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33480 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33480 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:bdbD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bdbD_3438065_3438733_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:bdbD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33480]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[genetic competence]]}},
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| {{SubtiWiki category|[[chaperones/ protein folding]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[ComK regulon]]}},
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| {{SubtiWiki regulon|[[SigE regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU33480
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| ===Phenotypes of a mutant ===
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| * loss of transformability [http://www.ncbi.nlm.nih.gov/sites/entrez/11744713 PubMed]
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| * sensitive to osmotic shock {{PubMed|22540663}}
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| * several proteins are absent from the membrane proteome of a ''[[bdbC]]-[[bdbD]]'' mutant: {{PubMed|22540663}}
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| ** the membrane proteins [[BglP]], [[TcyP]], [[SipU]], [[LytA]], and [[YxaI]] {{PubMed|22540663}}
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| ** the cytoplasmic or membrane-associated proteins [[GlkX]], [[ProA]], [[PyrAA]], [[PyrAB]], [[PyrH]], [[PyrE]], [[PyrF]], [[DegS]], [[YbxA]] {{PubMed|22540663}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33480&redirect=T BSU33480]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bdbDC.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14104]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** formation of thiol disulfide bonds in [[ComEC]] and [[ComGC]] (together with [[BdbC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11744713 PubMed]
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| * '''Protein family:''' DsbA subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):''' Ca (2+) [http://www.ncbi.nlm.nih.gov/sites/entrez/19535335 PubMed]
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' membrane, faced to the outer side of the membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/19535335 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33480&redirect=T BSU33480]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3EU3 3EU3] (reduced form), [http://www.rcsb.org/pdb/explore.do?structureId=3EU4 3EU4] (oxidized form) [http://www.ncbi.nlm.nih.gov/sites/entrez/19535335 PubMed]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O32218 O32218]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33480]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[bdbD]]-[[bdbC]]'' {{PubMed|11744713}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bdbD_3438065_3438733_-1 bdbD] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigE]] {{PubMed|12480901}}
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| * '''Regulation:'''
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| ** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|12480901}}
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| * '''Regulatory mechanism:'''
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| ** [[ComK]]: transcription activation {{PubMed|11744713}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 127 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 656 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>19535335, 11744713,15661011,11872755,16751195,11844773, 12480901 22540663 </pubmed>
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| [[Category:Protein-coding genes]]
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