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| * '''Description:''' bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase<br/><br/>
| | {{#bank: GcaD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gcaD''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''tms, tms26 ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || bifunctional N-acetylglucosamine-1-phosphate
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| uridyltransferase/glucosamine-1-phosphate acetyltransferase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || cell wall metabolism
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00500 gcaD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gcaD gcaD]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 5.65
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1368 bp, 456 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoVG]]'', ''[[prs]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00500 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00500 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00500 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:gcaD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gcaD_56352_57722_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gcaD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00500]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell wall synthesis]]}},
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| {{SubtiWiki category|[[biosynthesis of cell wall components]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU00500
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00500&redirect=T BSU00500]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gcaD-prs-ctc.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10113]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate (according to Swiss-Prot)
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00500&redirect=T BSU00500]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=1hm9 1HM9] (from ''Streptococcus pneumoniae'', 49% identity) {{PubMed|11118459}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P14192 P14192]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00500]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.23 2.7.7.23]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[gcaD]]-[[prs]]-[[ctc]]'' [http://www.ncbi.nlm.nih.gov/pubmed/8522540 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gcaD_56352_57722_1 gcaD] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8522540 PubMed]
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| * '''Regulation:'''
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| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 397 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1922 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| **pGP2596: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>1328164,8522540, 11118459, 21303765</pubmed>
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| [[Category:Protein-coding genes]]
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