|
|
| Line 1: |
Line 1: |
| * '''Description:''' triose phosphate isomerase, glycolytic/ gluconeogenic enzyme<br/><br/>
| | {{#bank: Tpi}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''tpi''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''tpiA''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || triosephosphate isomerase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33920 tpi]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Tpi Tpi]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=tpi tpi]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26,9 kDa, 4.79
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 759 bp, 253 amino acids
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pgm]]'', ''[[pgk]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33920 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:tpi_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tpiA_3479405_3480166_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tpi_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33920]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[carbon core metabolism]]}},
| |
| {{SubtiWiki category|[[phosphoproteins]]}},
| |
| [[most abundant proteins]]
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CggR regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU33920
| |
|
| |
| ===Phenotypes of a mutant ===
| |
| * essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519}}
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33920&redirect=T BSU33920]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10897]
| |
|
| |
| === Additional information===
| |
| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' triosephosphate isomerase family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
|
| |
| * '''Modification:''' phosphorylation on Ser-213 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:''' inhibited by 2-phosphoglycolate (in ''B. stearothermophilus'') [http://www.ncbi.nlm.nih.gov/sites/entrez/8580851 PubMed]
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[Tkt]]-[[Tpi]] {{PubMed|19193632}}
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33920&redirect=T BSU33920]
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1BTM 1BTM] (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, ''Geobacillus stearothermophilus'' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=48255 NCBI]
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P27876 P27876]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33920]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/5.3.1.1 5.3.1.1]
| |
|
| |
| === Additional information===
| |
| * extensive information on the structure and enzymatic properties of Tpi can be found at [http://www.proteopedia.org/wiki/index.php/Triose_Phosphate_Isomerase Proteopedia]
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
| ** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
| |
| ** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tpiA_3479405_3480166_-1 tpi] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11489127}}
| |
|
| |
| * '''Regulation:'''
| |
| ** expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
| |
| ** ''[[cggR]]'': induced by glycolytic substrates [[CggR]] {{PubMed|11489127}}
| |
| ** ''[[pgk]]'': constitutive {{PubMed|11489127}}
| |
|
| |
| * '''Regulatory mechanism:''' transcription repression by [[CggR]] {{PubMed|11489127}}
| |
|
| |
| * '''Additional information:'''
| |
| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 4265 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 5061 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2920 {{PubMed|21395229}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1493 {{PubMed|21395229}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2091 {{PubMed|21395229}}
| |
|
| |
| =Biological materials =
| |
| * '''Mutant:''' GP700 (cat), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
| |
|
| |
| * '''Expression vector:'''
| |
| ** pGP394 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
| |
| ** pGP89 (N-terminal Strep-tag, for [[SPINE]], expression in B. subtilis), available in [[Jörg Stülke]]'s lab
| |
| ** pGP1511 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed>12850135 11489127 17505547 8021172 17218307 8580851 19193632 21821766 23420519 15378759</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
