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| * '''Description:''' penicillin-binding protein X <br/><br/>
| | {{#bank: PbpX}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pbpX''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || penicillin-binding protein X
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| |style="background:#ABCDEF;" align="center"|'''Function''' || endopeptidase
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16950 pbpX]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 9.711
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1173 bp, 391 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[recA]]'', ''[[rny]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16950 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16950 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16950 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pbpX_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpX_1765859_1767034_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pbpX_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16950]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell wall synthesis]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigM regulon]]}},
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| {{SubtiWiki regulon|[[SigX regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16950
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| ===Phenotypes of a mutant ===
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| * increased sensitivity to lysozyme {{PubMed|21856855}}
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| * inactivation of ''[[pbpX]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16950&redirect=T BSU16950]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pbpX.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12642]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
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| ** localizes during sporulation to both asymmetric septa and the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16950&redirect=T BSU16950]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31773 O31773]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16950]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''pbpX'' {{PubMed|14762009}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpX_1765859_1767034_1 pbpX] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigM]] {{PubMed|18179421}}, [[SigX]] {{PubMed|14762009,15758244}}
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| * '''Regulation:'''
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| ** expression is reduced in a [[SigV]] mutant {{PubMed|21926231}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 491 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|21856855,21926231,22211522}}
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| <pubmed>14762009,,18957862,18179421 </pubmed>
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| [[Category:Protein-coding genes]]
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