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| * '''Description:''' scaffold of the [[germinosome]], required for clustering of germinant receptors in the spore inner membrane<br/><br/>
| | {{#bank: GerD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gerD''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || lipoprotein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[germination]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01550 gerD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GerD GerD]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 4.862
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 555 bp, 185 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[salA]]'', ''[[kbaA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01550 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:gerD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gerD_158515_159072_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gerD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01550]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[germination]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigF regulon]]}},
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| {{SubtiWiki regulon|[[SigG regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU01550
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| ===Phenotypes of a mutant ===
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| * defective in [[germination]] in response to nutrients {{PubMed|24530795}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01550&redirect=T BSU01550]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gerD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10644]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' required for clustering of germinant receptors in the spore inner membrane {{PubMed|21696470}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** has an N-terminal signal sequence and a signal for fixation to lipid {{PubMed|23335419}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** forms trimeric rope-like structures {{PubMed|24530795}}
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| ** [[GerD]] is part of the [[germinosome]] in the spore inner membrane {{PubMed|21696470}}
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| ** [[GerD]]-[[Lgt]] to fix [[GerD]] to diacylglycerol in the membrane {{PubMed|22493018}}
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| * '''[[Localization]]:'''
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| ** outer surface of the inner spore membrane {{PubMed|23335419,21696470}}
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| ** inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction [http://www.ncbi.nlm.nih.gov/sites/entrez/19332816 PubMed]
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01550&redirect=T BSU01550]
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| * '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=4O8W 4O8W] {{PubMed|24530795}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P16450 P16450]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01550]
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| * '''E.C. number:'''
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| === Additional information===
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| * 3,500 molecules are present per spore {{PubMed|23749970}}
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| =Expression and regulation=
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| * '''Operon:''' [[gerD]] {{PubMed|2517635}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gerD_158515_159072_-1 gerD] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigF]] {{PubMed|15699190}}, [[SigG]] {{PubMed|1906867,15699190}}
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| * '''Regulation:'''
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| ** expression level depends on sporulation conditions (medim composition, temperature) {{PubMed|22327596}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** 3,500 molecules are present per spore {{PubMed|23749970}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A677 ( ''gerD''::''erm''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A677&Search=1A677 BGSC]
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| ** 1G17 ( ''gerD''::''spec''), {{PubMed|15126458}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1G17&Search=1G17 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:''' available in [[Anne Moir]] lab
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| =Labs working on this gene/protein=
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| [[Anne Moir]], University of Sheffield, UK
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| =Your additional remarks=
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| =References=
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| <pubmed>1906867,19332816,17122337, 21725007,18556788, 19332816 2517635 21696470 22327596 22493018 23335419 23749970 21398556 24530795 </pubmed>
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| [[Category:Protein-coding genes]]
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