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| * '''Description:''' acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase) <br/><br/>
| | {{#bank: AcoC}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''acoC''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yfjI ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || acetoin utilization
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08080 acoC]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=AcoC AcoC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=acoC acoC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 6.524
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1194 bp, 398 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acoB]]'', ''[[acoL]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU08080 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU08080 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU08080 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:acoC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acoC_881049_882245_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:acoC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08080]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of specific carbon sources]]}},
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| {{SubtiWiki category|[[Sporulation#Sporulation_proteins/_other|sporulation proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AcoR regulon]]}},
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[SigL regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU08080
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU08080&redirect=T BSU08080]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acoABCL.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12560]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine (according to Swiss-Prot)
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| * '''Protein family:''' lipoyl-binding domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[BkdB]], [[OdhB]], [[PdhC]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| ** lipoic acid
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** ([[AcoA]]-[[AcoB]])-[[AcoC]]-[[AcoL]]
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| * '''[[Localization]]:''' Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU08080&redirect=T BSU08080]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31550 O31550]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08080]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.12 2.3.1.12]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[acoA]]-[[acoB]]-[[acoC]]-[[acoL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acoC_881049_882245_1 acoC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
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| * '''Regulation:'''
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| ** repressed by glucose ([[CcpA]]) {{PubMed|10666464}}
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| ** induced by acetoin ([[AcoR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
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| ** induced under anaerobic conditions ([[Fnr]]) {{PubMed|16428414}}
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| * '''Regulatory mechanism:'''
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| ** [[CcpA]]: transcription repression {{PubMed|10666464}}
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| ** [[AcoR]]: transcription activation (interaction with [[SigL]]-containing [[RNA polymerase]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
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| ** [[Fnr]]: indirect positive regulation via [[NarG]]-[[NarH]]-[[NarJ]]-[[NarI]] {{PubMed|16428414}}
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| * '''Additional information:'''
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| ** the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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| ** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 114 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 325 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 848 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Michel Debarbouille]], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>11274109,10368162,10666464,16479537,16428414 12884008 21815947 </pubmed>
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| [[Category:Protein-coding genes]]
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