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| * '''Description:''' alanine-anticapsin ligase <br/><br/>
| | {{#bank: BacD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''bacD''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywfE, ipa-83d''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || alanine-anticapsin ligase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the antibiotic bacilysin
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37710 bacD]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 52 kDa, 4.679
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1416 bp, 472 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bacE]]'', ''[[bacC]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37710 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37710 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37710 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywfE_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bacD_3870668_3872086_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:bacD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37710]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[miscellaneous metabolic pathways]]}},
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| {{SubtiWiki category|[[biosynthesis of antibacterial compounds]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[ScoC regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37710
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37710&redirect=T BSU37710]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10629]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis {{PubMed|23317005}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37710&redirect=T BSU37710]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3VMM 3VMM] {{PubMed|22407814}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39641 P39641]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37710]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.2.28 6.3.2.28]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[bacA]]-[[bacB]]-[[bacC]]-[[bacD]]-[[bacE]]-[[bacF]]'' {{PubMed|12372825}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bacD_3870668_3872086_-1 bacD] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|19801406}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[CodY]]: transcription repression {{PubMed|12372825,21709425}}
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| ** [[AbrB]]: transcription repression {{PubMed|12697329,21709425}}
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| ** [[ScoC]]: transcription repression {{PubMed|19801406}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>16030213,12372825,19352016,19801406 21948839 22407814 24702628 23317005,12697329,21709425,22298000</pubmed>
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| [[Category:Protein-coding genes]]
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