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| * '''Description:''' GTPase essential for ribosome 50S subunit assembly<br/><br/>
| | {{#bank: EngA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''yphC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' engA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || GTPase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || ribosome assembly
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22840 engA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.248
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1308 bp, 436 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gpsA]]'', ''[[ypzH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22840 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yphC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:yphC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22840]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| {{SubtiWiki category|[[GTP-binding proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU22840
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22840&redirect=T BSU22840]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11443]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Binds and hydrolyzes GTP and readily exchanges GDP for GTP
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| * '''Protein family:''' EngA subfamily (according to Swiss-Prot) Era/Obg family
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| * '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22840&redirect=T BSU22840]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2HJG 2HJG] (complex with GDP)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P50743 P50743]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22840]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 engA] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 225 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 935 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| ** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP846, available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Naotake Ogasawara]], Nara, Japan
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 19575570 21885683</pubmed>
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| ==Original publications==
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| <pubmed>12427945, 16997968,16894162 16682769 23056455</pubmed>
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| [[Category:Protein-coding genes]]
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