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| * '''Description:''' NAD kinase <br/><br/>
| | {{#bank: NadF}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''nadF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjbN, ppnK''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || inorganic polyphosphate/ATP-NAD kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || generation of NADP from NAD
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11610 nadF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=NadF NadF]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.991
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 798 bp, 266 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sasB]]'', ''[[yjbO]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11610 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yjbN_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ppnKA_1237660_1238460_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:nadF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11610]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis of cofactors]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU11610
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11610&redirect=T BSU11610]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13143]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + NAD<sup>+</sup> = ADP + NADP<sup>+</sup> (according to Swiss-Prot)
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| * '''Protein family:''' NAD kinase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:''' allosterically activated by quinolinic acid
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11610&redirect=T BSU11610]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31612 O31612]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11610]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.23 2.7.1.23]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[yjbL]]-[[sasB]]-[[nadF]]-[[yjbO]]'' {{PubMed|18067544}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ppnKA_1237660_1238460_1 nadF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' expressed during exponential growth
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 88 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 405 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A825 ( ''nadF''::''erm''), {{PubMed|12682299}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A825&Search=1A825 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12897004,18067544, </pubmed>
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| [[Category:Protein-coding genes]]
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