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| * '''Description:''' transketolase <br/><br/>
| | {{#bank: Tkt}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''tkt''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''tktA ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transketolase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17890 tkt]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Tkt Tkt]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=tkt tkt]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 72 kDa, 4.803
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2001 bp, 667 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynzC]]'', ''[[sirA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17890 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:tkt_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tkt_1919861_1921864_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tkt_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17890]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU17890
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17890&redirect=T BSU17890]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tkt.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11247]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)
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| * '''Protein family:''' transketolase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:''' phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed]
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| * '''[[Cofactors]]:''' thiamine pyrophosphate
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:''' [[Tkt]]-[[Tpi]] {{PubMed|19193632}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17890&redirect=T BSU17890]
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| * '''Structure:''' [[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3K95 3K95]], complex with thiamine diphosphate, from ''B. anthracis''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P45694 P45694]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17890]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.2.1.1 2.2.1.1]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''tkt'' {{PubMed|9068642}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tkt_1919861_1921864_1 tkt] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| * '''Additional information:'''
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 7856 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 18478 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' BS4530 (aphA3), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| ** pGP94 (N-terminal Strep-tag, for [[SPINE]], expression in B. subtilis, in [[pGP380]]), available in [[Stülke]] lab
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| ** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP820, available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| * '''FLAG-tag construct:''' GP1406 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>9924800 </pubmed>
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| ==Original publications==
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| <pubmed>14651647,16493705, 19603213 9068642 16143417 15375635 19193632 23568212 23965678 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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