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| * '''Description:''' surfactin synthetase / competence <br/><br/>
| | {{#bank: SrfAB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''srfAB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''comL ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || surfactin synthetase / competence
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| |style="background:#ABCDEF;" align="center"|'''Function''' || antibiotic synthesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03490 srfAB]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 400 kDa, 4.903
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 10761 bp, 3587 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[srfAA]]'', ''[[comS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03490 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03490 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03490 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:srfAB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=srfAB_387744_398495_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:srfAB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03490]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[miscellaneous metabolic pathways]]}},
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| {{SubtiWiki category|[[biosynthesis of antibacterial compounds]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Abh regulon]]}},
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[ComA regulon]]}},
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| {{SubtiWiki regulon|[[PerR regulon]]}},
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| {{SubtiWiki regulon|[[Spx regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU03490
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03490&redirect=T BSU03490]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/srfAA-srfAB-comS-srfAC-srfAD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10169]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| ** phosphorylation on Ser-999 AND Ser-2045 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| ** phosphorylated on Arg-1378 {{PubMed|22517742}}
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03490&redirect=T BSU03490]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q04747 Q04747]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU03490]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[srfAA]]-[[srfAB]]-[[comS]]-[[srfAC]]-[[srfAD]]'' {{PubMed|1715856}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=srfAB_387744_398495_1 srfAB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1715856}}
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| * '''Regulation:'''
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8830686 PubMed]
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| ** repressed during hydrogen peroxide stress ([[PerR]]) {{PubMed|16166527}}
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| ** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}
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| * '''Regulatory mechanism:'''
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8830686 PubMed]
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| ** [[ComA]]: transcription activation {{PubMed|1715856,16091051}}
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| ** [[PerR]]: transcription activation {{PubMed|16166527}}
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| ** [[Spx]]: transcription repression {{PubMed|12642660}}
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| ** [[Abh]]: transcription repression{{PubMed|20817675}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>20735481 </pubmed>
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| ==Original publications==
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| <pubmed>17227471,16166527,8288534,10960106, 12642660,8830686,18763711 17218307, 17190806 1715856 16091051 22517742 8355610 22511326, 20817675 </pubmed>
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| [[Category:Protein-coding genes]]
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