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| * '''Description:''' arginase <br/><br/>
| | {{#bank: RocF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rocF''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || arginase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || arginine utilization
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40320 rocF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=rocF rocF]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 4.932
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 888 bp, 296 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phrG]]'', ''[[rocE]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40320 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rocF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=argI_4141711_4142601_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rocF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40320]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of amino acids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AhrC regulon]]}},
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[RocR regulon]]}},
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| {{SubtiWiki regulon|[[SigL regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU40320
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40320&redirect=T BSU40320]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocDEF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10932]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' L-arginine + H<sub>2</sub>O = L-ornithine + urea (according to Swiss-Prot)
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| * '''Protein family:''' arginase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:''' phosphorylated on Ser-68 {{PubMed|20509597}}
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40320&redirect=T BSU40320]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2CEV 2CEV] (from ''Bacillus caldevelox'', 71% identity) {{PubMed|10196128}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39138 P39138]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40320]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.5.3.1 3.5.3.1]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[rocD]]-[[rocE]]-[[rocF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7540694 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=argI_4141711_4142601_-1 rocF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7540694 PubMed]
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| * '''Regulation:'''
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| ** induced by arginine ([[RocR]], [[AhrC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7540694 PubMed]
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[RocR]], [[AhrC]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/7540694 PubMed]
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| ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 126 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 13789 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' GP655, aphA3, available in the [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12618455, 20509597,14651647,12618455,7540694, 23625224 10196128 </pubmed>
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| [[Category:Protein-coding genes]]
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