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| * '''Description:''' [[ptsH|HPr]] [[protein kinases and phosphatases|kinase]]/ phosphorylase<br/><br/>
| | {{#bank: HprK}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''hprK''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ptsK, yvoB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[ptsH|HPr]] [[protein kinases and phosphatases|kinase]]/ phosphorylase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || carbon catabolite repression,<br/>phosphorylation of [[ptsH|HPr]] and [[Crh]] proteins at Ser46
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35000 hprK]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=HPrK HprK]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=hprK hprK]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 4.906
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 930 bp, 310 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lgt]]'', ''[[nagA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35000 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:hprK_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hprK_3594242_3595174_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:hprK_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35000]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU35000
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| ===Phenotypes of a mutant ===
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| no carbon catabolite repression
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35000&redirect=T BSU35000]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14125]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + [[ptsH|HPr]] = ADP + P-Ser-HPr (according to Swiss-Prot)
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| * '''Protein family:''' HPrK/P family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** HprK-[[PtsH| HPr]]
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| ** HprK-[[Crh]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12009882 PubMed]
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35000&redirect=T BSU35000]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1KKM 1KKM] (complex of Lactobacillus casei HprK with B. subtilis HPr-Ser-P), [http://www.rcsb.org/pdb/explore.do?structureId=1KKL 1KKL] (complex of Lactobacillus casei HprK with B. subtilis HPr)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34483 O34483]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35000]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[hprK]]-[[lgt]]-[[yvoD]]-[[yvoE]]-[[yvoF]]'' {{PubMed|9465101}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hprK_3594242_3595174_-1 hprK] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|23667565}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 398 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 875 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' GP202 (spc) {{PubMed|12055300}}, GP858 (aphA3) {{PubMed|18757537}}, GP82 (cat) {{PubMed|21992469}}, all available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP642, available in [[Jörg Stülke]]'s lab
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| ** for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP650, available in [[Jörg Stülke]]'s lab
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| ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP205, available in [[Jörg Stülke]]'s lab
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| ** for expression, purification of the N-terminal in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP218, available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:''' pGP201 (in [[pAC5]], {{PubMed|12055300}}) available in [[Stülke]] lab, pGP202 (in [[pAC6]], {{PubMed|12055300}}) available in [[Jörg Stülke]]'s lab
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:''' available in [[Jörg Stülke]]'s lab {{PubMed|12055300}}
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| =Labs working on this gene/protein=
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| [[Josef Deutscher]], Paris-Grignon, France
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| [[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| [[Wolfgang Hillen]], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
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| [[Anne Galinier]], University of Marseille, France
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>12837773 15023355 18628769 </pubmed>
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| ==General Analysis, Physiology==
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| <pubmed>9570401 9465101 12123463 18757537 22001508 23667565</pubmed>
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| ==Structural Analysis of HPrK==
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| <pubmed>11904409 12589763 12359875 17878158 </pubmed>
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| ==Enzymatic Properties, Mutation Analysis==
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| <pubmed>12359880 11483496 12055300 10636874 12411438 11796714 12009882 12779331 </pubmed>
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| ==HprK as a Target For Antimicrobial Compounds==
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| <pubmed>15084125, </pubmed>
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| [[Category:Protein-coding genes]]
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