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| * '''Description:''' fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme<br/><br/>
| | {{#bank: FbaA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''fbaA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''fba, fba1, tsr ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || fructose-1,6-bisphosphate aldolase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37120 fbaA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=fbaA fbaA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30,2 kDa, 5.03
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 855 bp, 285 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywjH]]'', ''[[spo0F]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37120 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37120 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37120 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:fbaA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fbaA_3808512_3809369_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:fbaA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37120]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37120
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| ===Phenotypes of a mutant ===
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| * essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37120&redirect=T BSU37120]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fbaA-ywjH.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10412]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)
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| * '''Protein family:''' class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[FbaB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| ** Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/15125960 PubMed]
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| ** specific activity: 0.049 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}}
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| * '''[[Domains]]:'''
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| ** 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
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| * '''Modification:''' phosphorylation on Thr-212 and Thr-234 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| * '''[[Cofactors]]:''' Zn2+ (Metalloenzyme)
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| * '''Effectors of protein activity:'''
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| ** Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate [http://www.ncbi.nlm.nih.gov/pubmed/24624 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/15125960 PubMed]
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| ** Activated by NH4+ [http://www.ncbi.nlm.nih.gov/sites/entrez/15125960 PubMed]
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37120&redirect=T BSU37120]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3Q94 3Q94] (from ''Bacillus anthracis'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P13243 P13243]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37120]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.2.13 4.1.2.13]
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| === Additional information===
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| * Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
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| * extensive information on the structure and enzymatic properties of FbaA can be found at [http://www.proteopedia.org/wiki/index.php/Fructose_Bisphosphate_Aldolase Proteopedia]
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| =Expression and regulation=
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| * '''Operon:'''
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| **''fbaA'' {{PubMed|11489127}}
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| ** ''[[fbaA]]-[[ywjH]]'' {{PubMed|11489127}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fbaA_3808512_3809369_-1 fbaA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** constitutively expressed {{PubMed|11489127}}
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| ** strongly repressed in response to glucose starvation in M9 medium {{PubMed|23033921}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** The mRNA has a long 5' leader region. This may indicate RNA-based regulation {{PubMed|20525796}}
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 15625 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 45663 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * GP591 (''fbaA''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
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| * GP596 (''fbaA''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
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| * '''Expression vector:'''
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| ** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1423, available in [[Jörg Stülke]]'s lab
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| ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP88, available in [[Jörg Stülke]]'s lab
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| ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP395, available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:''' pGP601 (in [[pAC6]])
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>17218307, 15125960, 24624 16843441 11489127 20525796 23420519 23033921 24571712 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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