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| * '''Description:''' isochorismate synthase <br/><br/>
| | {{#bank: DhbC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''dhbC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || isochorismate synthase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the siderophore bacillibactin
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31990 dhbC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=DhbC DhbC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 5.168
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1194 bp, 398 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dhbE]]'', ''[[dhbA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31990 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31990 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31990 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:dhbC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dhbC_3290289_3291485_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:dhbC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31990]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[acquisition of iron]]}},
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| {{SubtiWiki category|[[iron metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[Fur regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU31990
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31990&redirect=T BSU31990]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dhbACEBF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11242]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Chorismate = isochorismate (according to Swiss-Prot)
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| * '''Protein family:''' isochorismate synthase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:''' phosphorylation on Ser-271 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31990&redirect=T BSU31990]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3OS6 3OS6], (from ''B. anthracis'', 56% identity, 70% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P45744 P45744]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31990]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/5.4.4.2 5.4.4.2]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[dhbA]]-[[dhbC]]-[[dhbE]]-[[dhbB]]-[[dhbF]]'' {{PubMed|8921902}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dhbC_3290289_3291485_-1 dhbC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8550523}}
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| * '''Regulation:'''
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| ** induced by iron starvation ([[Fur]]) {{PubMed|12354229}}
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| * '''Regulatory mechanism:'''
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| ** [[Fur]]: transcription repression {{PubMed|12354229}}
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| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
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| * '''Additional information:'''
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| ** the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 9037 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 3893 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>8224884,11112781 ,11790741,12354229,17218307, 8921902 8550523 20817675 21815947</pubmed>
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| [[Category:Protein-coding genes]]
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