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| * '''Description:''' adenylyl-sulfate kinase <br/><br/>
| | {{#bank: CysC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cysC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylnC ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || adenylyl-sulfate kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || sulfate reduction and activation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15600 cysC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=cysC cysC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.083
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 591 bp, 197 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sat]]'', ''[[ylnD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15600 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:cysC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysC_1633369_1633962_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cysC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15600]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[sulfur metabolism]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CymR regulon]]}},
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| {{SubtiWiki regulon|[[S-box]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU15600
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15600&redirect=T BSU15600]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13379]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate (according to Swiss-Prot)
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| * '''Protein family:''' APS kinase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15600&redirect=T BSU15600]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3CR8 3CR8] (from ''Thiobacillus denitrificans'', 46% identity, 62% similarity) {{PubMed|19770499}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34577 O34577]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15600]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.25 2.7.1.25]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[cysH]]-[[cysP]]-[[sat]]-[[cysC]]-[[ylnD]]-[[ylnE]]-[[ylnF]]'' {{PubMed|11004190}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysC_1633369_1633962_1 cysC] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|11004190}}
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| * '''Regulation:'''
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| ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
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| ** repressed in the presence of cysteine ([[CymR]])
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| ** induced by methionine starvation ([[S-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[S-box]]: transcription termination/ antitermination, the [[S-box]] [[riboswitch]] binds S-adenosylmethionine resulting in termination [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed]
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| ** [[CymR]]: transcription repression
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1080 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1030 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France
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| =Your additional remarks=
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| =References=
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| <pubmed>16267287,11004190,12107147,18039762 </pubmed>
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| [[Category:Protein-coding genes]]
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