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| * '''Description:''' proline dehydrogenase (L-proline, NAD) <br/><br/>
| | {{#bank: PutB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''putB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ycgM''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || proline dehydrogenase (L-proline, NAD)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || proline utilization
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03200 putB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=putB putB]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 7.291
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 909 bp, 303 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgL]]'', ''[[putC]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03200 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03200 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03200 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ycgM_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycgM_344551_345462_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:putB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03200]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of amino acids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[PutR regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU03200
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| ===Phenotypes of a mutant ===
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| * no growth with proline as single source of carbon or nitrogen {{PubMed|21840319}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03200&redirect=T BSU03200]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ycgMN.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12011]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)
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| * '''Protein family:''' proline dehydrogenase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[FadM]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03200&redirect=T BSU03200]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P94390 P94390]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU03200]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.5.99.8 1.5.99.8]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[putB]]-[[putC]]-[[putP]]'' {{PubMed|21840319, 22139509}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycgM_344551_345462_1 putB] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|21840319,21964733,22139509}}
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| * '''Regulation:'''
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| ** induced in the presence of proline ([[PutR]]) {{PubMed|21840319,22139509}}
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
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| ** strongly induced in response to glucose starvation in M9 medium {{PubMed|23033921}}
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| * '''Regulatory mechanism:'''
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| ** [[PutR]]: transcription activation {{PubMed|21840319,21964733,22139509}}
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| ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** [[CodY]]: transcription repression, displacement of [[PutR]] {{PubMed|21840319}}
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| * '''Additional information:'''
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| ** overexpressed in a ''[[pnpA]]'' mutant {{PubMed|14976255}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>14976255,21840319,14651647,12618455,23033921,21964733,22139509</pubmed>
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| [[Category:Protein-coding genes]]
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