|
|
| Line 1: |
Line 1: |
| * '''Description:''' activator of [[RsbT]] kinase activity, [[stressosome]] sensor protein
| | {{#bank: RsbR}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''rsbR''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycxR, rsbRA''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || activator of [[RsbT]] kinase activity, [[stressosome]] sensor protein
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigB]] activity
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04670 rsbR]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RsbR RbsR]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=rsbR rsbR]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 4.731
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 822 bp, 274 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ndoA]]'', ''[[rsbS]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU04670 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU04670 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU04670 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:rsbR_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbRA_519408_520232_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rsbR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04670]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[sigma factors and their control]]}},
| |
| {{SubtiWiki category|[[phosphoproteins]]}},
| |
| [[most abundant proteins]]
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
|
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU04670
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04670&redirect=T BSU04670]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rsbRSTUVW-sigB-rsbX.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11090]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
| ** positive regulator of [[YtvA]]-dependent light activation of the [[SigB]] stress response {{PubMed|22287516}}
| |
| ** phosphorylated [[RsbR]] activates the kinase activity of [[RsbT]] {{PubMed|23320651}}
| |
|
| |
| * '''Protein family:'''
| |
|
| |
| * '''Paralogous protein(s):''' [[RsbRB]], [[RsbRC]], [[RsbRD]], [[YtvA]]
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
| ** RsbRA is composed of an N-terminal nonheme globin domain and a highly conserved C-terminal STAS (Sulphate Transporter and AntiSigma factor antagonist) domain. The C-terminal STAS domain is the target of the serine/threonine-specific kinase [[RsbT]] (see below).
| |
|
| |
| * '''Modification:''' phosphorylation on Thr-171 and Thr-205 by [[RsbT]] {{PubMed|21362065}}
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[RsbX]]-[[RsbR]] {{PubMed|15466036}}
| |
| ** [[RsbR]]-[[RsbT]] {{PubMed|21362065}}
| |
| ** [[RsbR]]-[[RsbS]]
| |
| ** [[YtvA]]-[[RsbR]] {{PubMed|11157946}}
| |
|
| |
| ** component of the [[stressosome]]
| |
|
| |
| * '''[[Localization]]:'''
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04670&redirect=T BSU04670]
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2BNL 2BNL] (N-terminal domain), [http://www.rcsb.org/pdb/explore.do?structureId=3VY9 3VY9] (complete stressosome)
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P42409 P42409]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04670]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''[[rsbR]]-[[rsbS]]-[[rsbT]]-[[rsbU]]-[[rsbV]]-[[rsbW]]-[[sigB]]-[[rsbX]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed]
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbRA_519408_520232_1 rsbR] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed]
| |
|
| |
| * '''Regulation:''' constitutively expressed {{PubMed|20019076}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| * [[Bill Haldenwang]], San Antonio, USA
| |
| * [[Chet Price]], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]
| |
| * [[Rick Lewis]], Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/r.lewis homepage]
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed>19704888 16319496 20658979 23416074 </pubmed>
| |
|
| |
| ==Original Articles==
| |
| <pubmed>8002610,8682769,8682789, 17726680,10781545,15583165, 8824586,10329124,17158665, 9179850,8808936,15312768, 11244072,15342582,15378759, 12950928, 15466036, 9179850, 8955331, 18832644 ,17726680 ,17218307 20019076 21602359 23320651,21362065,20935101,22287516,22609918 24599254 </pubmed>
| |
| [[Category:Protein-coding genes]]
| |
