TagD

• Description: glycerol-3-phosphate cytidylyltransferase
  
  

Gene name	tagD
Synonyms
Essential	yes PubMed
Product	glycerol-3-phosphate cytidylyltransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagD
Metabolic function and regulation of this protein in SubtiPathways: TagD
MW, pI	15 kDa, 5.672
Gene length, protein length	387 bp, 129 aa
Immediate neighbours	tagE, tagA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35740

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU35740

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol (according to Swiss-Prot)

• Protein family: cytidylyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TagD-TagD PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35740

• Structure: 1N1D (complex with CDP-glycerol) PubMed, 1COZ

• UniProt: P27623

• KEGG entry: [3]

• E.C. number: 2.7.7.39

Expression and regulation

• Operon: tagD-tagE-tagF PubMed

• Expression browser: tagD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by phosphate (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 604 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2215 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>9683503,9457886,11673441,10508782,2507871,12950927, 7581998 20525796</pubmed>