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| * '''Description:''' tryptophanyl-tRNA synthetase <br/><br/>
| | {{#bank: TrpS}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''trpS''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || tryptophanyl-tRNA synthetase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || translation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11420 trpS]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=trpS trpS]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 6.542
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 990 bp, 330 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbA]]'', ''[[oppA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11420 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:trpS_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:trpS_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11420]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[T-box]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU11420
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11420&redirect=T BSU11420]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpS.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10799]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)
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| * '''Protein family:''' class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11420&redirect=T BSU11420]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3PRH 3PRH] (Val144Pro mutant) {{PubMed|21224416}}
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=1BM2 1BM2] (complex with tryptophan, ''Geobacillus stearothermophilus'')
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=1MAW 1MAW] (complex with ATP, ''Geobacillus stearothermophilus'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P21656 P21656]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11420]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/6.1.1.2 6.1.1.2]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''trpS'' {{PubMed|8915527}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 trpS] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| ** induced by tryptophan limitation ([[T-box]]) {{PubMed|8915527}}
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| * '''Regulatory mechanism:'''
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| ** [[T-box]]: transcriptional antitermination in the absence of tryptophan (upon tRNA-binding to the mRNA leader region) {{PubMed|8915527}}
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| * '''Additional information:'''
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| ** A [[ncRNA]] is predicted between ''[[trpS]]'' and ''[[oppA]]'' {{PubMed|20525796}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>19258532,8932308,8915527, 21224416 20525796</pubmed>
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| [[Category:Protein-coding genes]]
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