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| * '''Description:''' [[ABC transporter]] for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with [[YusV]] as ATPase <br/><br/>
| | {{#bank: FeuA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''feuA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[ABC transporter]] for the siderophores Fe-enterobactin <br/>and Fe-bacillibactin (binding protein)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[acquisition of iron]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01630 feuA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FeuA FeuA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=feuA feuA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 8.018
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 951 bp, 317 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[feuB]]'', ''[[btr]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01630 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:feuA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=feuA_182370_183323_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:feuA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01630]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ABC transporters]]}},
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| {{SubtiWiki category|[[acquisition of iron]]}},
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| {{SubtiWiki category|[[iron metabolism]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Btr regulon]]}},
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| {{SubtiWiki regulon|[[CitB regulon]]}},
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| {{SubtiWiki regulon|[[Fur regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU01630
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01630&redirect=T BSU01630]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/feuABC-ybbA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10835]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/biological activity:'''
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| Substrate binding protein for the triscatecholate siderophores ferri-bacillibactin and ferri-enterobactin and part of the [[FeuA]]-[[FeuB]]-[[FeuC]]-[[YusV]] ATP-binding cassette-type transporter. Furthermore ferric complexes of L-norepinephrine are bound by this protein.
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| * '''Protein family:''' Fe/B12 periplasmic-binding domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).
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| * '''Modification:'''
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| ** FeuA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure {{PubMed|22303020}}
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[YusV]]-([[FeuB]]-[[FeuC]])-[[FeuA]] {{PubMed|10092453}}
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| * '''[[Localization]]:'''
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| ** associated to the membrane (lipoprotein) {{PubMed|22303020}}
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| ** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01630&redirect=T BSU01630]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PHZ 2PHZ], [http://www.rcsb.org/pdb/explore.do?structureId=2WI8 2WI8], [http://www.rcsb.org/pdb/explore.do?structureId=2WHY 2WHY] (complex with ferri-bacillibactin), [http://www.rcsb.org/pdb/explore.do?structureId=2XUZ 2XUZ] (complex with ferri-enterobactin), [http://www.rcsb.org/pdb/explore.do?structureId=2XV1 2XV1] (complex with ferric mecam)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P40409 P40409]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01630]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[feuA]]-[[feuB]]-[[feuC]]-[[ybbA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=feuA_182370_183323_-1 feuA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17725565 PubMed]
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| * '''Regulation:'''
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| ** repressed in the presence of iron ([[Fur]], [[CitB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
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| ** induced in the presence of iron chelators bacillibactin or enterobactin ([[Btr]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17725565 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Fur]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
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| ** [[CitB]]: binding to the iron responsive element in the absence of iron
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| ** [[Btr]]: transcription activation in the presence of the co-activators bacillibactin or enterobactin [http://www.ncbi.nlm.nih.gov/sites/entrez/17725565 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|21802011}}
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| <pubmed>20086155,19746494,19673474,14563870,16672620,10092453,16889643,17725565,12354229,18957862 18763711 22303020 </pubmed>
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| [[Category:Protein-coding genes]]
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