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| * '''Description:''' repressor of ''[[comK]]'', nucleoid associated protein that serves to help repress expression of A+T-rich genes, many of which appear to have been acquired by horizontal gene transfer <br/><br/>
| | {{#bank: Rok}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rok''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykuW ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of genetic competence
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14240 rok]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=Rok Rok]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 9.701
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 573 bp, 191 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuV]]'', ''[[yknT]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14240 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14240 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14240 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rok_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rok_1493787_1494362_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rok_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14240]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[genetic competence]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[Rok regulon]]}},
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| {{SubtiWiki regulon|[[SinR regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The [[Rok regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU14240
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| ===Phenotypes of a mutant ===
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| higher excision rate of the conjugative transposon ICEBs1 {{PubMed|21085634}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14240&redirect=T BSU14240]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rok.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13307]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' binds to extended regions of the ''B. subtilis'' genome that are characterized by a high A+T content and are known or believed to have been acquired by horizontal gene transfer {{PubMed|21085634}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14240&redirect=T BSU14240]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34857 O34857]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14240]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''rok'' {{PubMed|12354229}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rok_1493787_1494362_1 rok] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| ** repressed by [[Rok]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| ** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| ** [[Rok]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19995980 </pubmed>
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| ==Original Publications==
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| '''Additional publications:''' {{PubMed|22524514,22412392}}
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| <pubmed>11849533,15819619,15743949, 21097620,17493123,14651647,12354229 21085634 22447908 </pubmed>
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| [[Category:Protein-coding genes]]
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