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| * '''Description:''' anti-repressor, antagonist of [[SinR]] and [[SlrR]], involved in control of flagellar genes and ''[[epsA|eps]]'' and ''[[tapA]]-[[sipW]]-[[tasA]]'' operons <br/><br/>
| | {{#bank: SlrA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''slrA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || antagonist of [[SinR]] and [[SlrR]]
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[biofilm formation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38229 slrA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SlrA SlrA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=slrA slrA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6.1 kDa, 9.992
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 156 bp, 52 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywcC]]'', ''[[ywcB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38229 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38229 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38229 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywcC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=slrA_3923319_3923477_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:slrA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38229]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[transition state regulators]]}},
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| {{SubtiWiki category|[[biofilm formation]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[YwcC regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU38229
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:'''
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| === Additional information===
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| * This gene was only recently identified. [http://www.ncbi.nlm.nih.gov/sites/entrez/18647168 PubMed]
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| * SlrA overexpression results in reduced expression of the [[SigD regulon]] and in enhanced expression of the [[SinR regulon]], this can be suppressed by inactivation of either ''[[remA]]'', ''[[remB]]'', ''[[slrR]]'', or ''[[sinR]]'' {{PubMed|22329926}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' binding of [[SinR]] resulting in induction of [[SinR]]-repressed genes and operons {{PubMed|19788541}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[SinI]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' [[SlrR]] inhibits activity of [[SlrA]] (i. e. binding to [[SinR]]) {{PubMed|19788541}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SlrA]]-[[SinR]] {{PubMed|19788541}}, K(D) 10.6 nM {{PubMed|23430750}}
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| ** [[SlrA]]-[[SlrR]] {{PubMed|19788541}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P0C8M5 P0C8M5]
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| * '''KEGG entry:''' no entry
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[slrA]]-[[ywcB]]-[[ywcA]]''
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| ** ''[[slrA]]-[[ywcB]]''
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| ** ''[[slrA]]''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=slrA_3923319_3923477_1 slrA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[YwcC]]: transcription repression {{PubMed|18647168,19788541}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|22329926}}
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| <pubmed>18647168, 19788541, 23430750</pubmed>
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| [[Category:Protein-coding genes]]
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