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| * '''Description:''' transcriptional regulator (repressor or activator) of a subset of [[sigE|sigma-E]]-dependent genes <br/><br/>
| | {{#bank: SpoIIID}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spoIIID''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of mother cell gene expression
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36420 spoIIID]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=spoIIID spoIIID]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 10 kDa, 9.496
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 279 bp, 93 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mbl]]'', ''[[usd]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36420 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spoIIID_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIID_3748421_3748702_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spoIIID_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36420]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigE regulon]]}}
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| =The [[SpoIIID regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36420
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/usd-spoIIID-mbl-flhOP.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10408]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' binds its DNA targets as a monomeric protein (possible due to the two DNA-binding motifs) {{PubMed|20061473}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:''' two DNA-binding regions: HTH at the N-terminus and a basic region near the C-terminus {{PubMed|20061473}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=2L0K 2L0K] (complex with DNA)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P15281 P15281]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36420]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[usd]]-[[spoIIID]]-[[mbl]]-[[flhO]]-[[flhP]]'' {{PubMed|9023218,7836311}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIID_3748421_3748702_-1 spoIIID] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|9023218}}
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| * '''Regulation:'''
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| ** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|9023218}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>15383836,1469717,7896717,1900494,8231808,9023218,2112673,1691789,10788508,1744038,7966271,2514119,1518043,17693505,9006059, 20061473 17890309 17693499 7836311 24584501 22463703</pubmed>
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| [[Category:Protein-coding genes]]
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