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| * '''Description:''' transcriptional activator of the ''[[alsS]]-[[alsD]]'' operon<br/><br/>
| | {{#bank: AlsR}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''alsR''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional activator([[LysR family]])
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of acetoin synthesis (''[[alsS]]-[[alsD]]'')
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36020 alsR]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=alsR alsR]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 7.288
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 906 bp, 302 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[alsS]]'', ''[[ywrK]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36020 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36020 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36020 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:alsR_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alsR_3711498_3712406_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:alsR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36020]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The [[AlsR regulon]]:=
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| * ''[[alsS]]-[[alsD]]''
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36020
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| ===Phenotypes of a mutant ===
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| no acetoin production
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10470]
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| === Additional information===
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| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
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| * Inactivation of ''[[alsR]]'', ''[[sigD]]'' and ''[[sigW]]'' increases competitive fitness of ''Bacillus subtilis'' under non-sporulating conditions {{PubMed|22344650}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' binds the promoter region of the ''[[alsS]]-[[alsD]]'' operon in the presence of acetate, activates transcription
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| * '''Protein family:''' [[LysR family]] of transcription factors
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| ** K(D) value for the binding site in the ''[[alsS]]'' promoter region: 33.5 nM {{PubMed|22178965}}
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q04778 Q04778]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36020]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alsR_3711498_3712406_1 alsR] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' AMBs3 (spc), available in the [[Stülke]] lab
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| ** 1A978 ( ''alsR''::''cat''), {{PubMed|7685336}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A978&Search=1A978 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Elisabeth Härtig]], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 23046954</pubmed>
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| ==Original publications==
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| <pubmed>7685336, 21821766 22344650 23576037 22178965 23695583 23836140 </pubmed>
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| [[Category:Protein-coding genes]]
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