TagU

• Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
  
  

Gene name	tagU
Synonyms	lytR
Essential	no
Product	phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function	transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagU
Interactions involving this protein in SubtInteract: TagU
MW, pI	34 kDa, 9.527
Gene length, protein length	918 bp, 306 aa
Immediate neighbours	lytA, mnaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon, SigX regulon

The gene

Basic information

• Locus tag: BSU35650

Phenotypes of a mutant

• a tagU mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

• BsubCyc: BSU35650

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LytR-Cps2a-Psr family PubMed

• Paralogous protein(s): TagT, TagV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mg(2+) PubMed

• Effectors of protein activity:

• Interactions:
  • MreB-TagU PubMed

• Localization:
  • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed

Database entries

• BsubCyc: BSU35650

• Structure: 3MEJ (TagT, 36% identity, 69% similarity)

• UniProt: Q02115

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tagU PubMed

• Expression browser: tagU PubMed

• Sigma factor: SigA PubMed, SigM PubMed, SigX PubMed

• Regulation:

• Regulatory mechanism:
  • LytR: autorepression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>9636707,1357079 , 19099556 21964069 </pubmed>