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| * '''Description:''' endolevanase <br/><br/>
| | {{#bank: LevB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''levB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yveB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || endolevanase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || levan degradation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34460 levB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=levB levB]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 58 kDa, 4.993
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1548 bp, 516 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacB]]'', ''[[yveA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34460 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yveB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levB_3537507_3539057_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:levB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34460]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of specific carbon sources]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[DegU regulon]]}},
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| {{SubtiWiki regulon|[[SacY regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU34460
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacB-yveBA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12430]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i (according to Swiss-Prot)
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| * '''Protein family:''' glycosyl hydrolase 32 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O07003 O07003]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34460]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.64 3.2.1.64]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[sacB]]-[[levB]]-[[yveA]]'' {{PubMed|11739774}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levB_3537507_3539057_1 levB] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|2428811}}
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| * '''Regulation:'''
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| ** induction by sucrose (at high concentration) ([[SacY]]) {{PubMed|3039303}}
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| * '''Regulatory mechanism:'''
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| ** [[SacY]]: binding to a [[RNA switch]] (transcriptional antitermination) {{PubMed|3039303}}
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| ** [[DegU]]-P: transcription activation {{PubMed|2428811}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>11739774 2428811 3039303</pubmed>
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| [[Category:Protein-coding genes]]
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