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| * '''Description:''' similar to glycosyltransferase <br/><br/>
| | {{#bank: EpsF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''epsF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yveP ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' ||
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| |style="background:#ABCDEF;" align="center"| '''Product''' || unknown
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34320 epsF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=epsF epsF]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 6.934
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1152 bp, 384 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[epsG]]'', ''[[epsE]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34320 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yveP_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=epsF_3523270_3524424_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:epsF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34320]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biofilm formation]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[EAR riboswitch]]}},
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| {{SubtiWiki regulon|[[RemA regulon]]}},
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| {{SubtiWiki regulon|[[SinR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU34320
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/eps.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11864]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' glycosyltransferase 1 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P71055 P71055]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34320]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[epsA]]-[[epsB]]-[[epsC]]-[[epsD]]-[[epsE]]-[[epsF]]-[[epsG]]-[[epsH]]-[[epsI]]-[[epsJ]]-[[epsK]]-[[epsL]]-[[epsM]]-[[epsN]]-[[epsO]]'' {{PubMed|15661000}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=epsF_3523270_3524424_-1 epsF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15661000}}
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| * '''Regulation:'''
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| ** repressed by [[SinR]] {{PubMed|15661000}}
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| * '''Regulatory mechanism:'''
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| ** [[SinR]]: transcription anti-activation (prevents binding of [[RemA]]) {{PubMed|23646920}}
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| ** [[RemA]]: transcription activation {{PubMed|23646920}}
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| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
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| * '''Additional information:'''
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| ** induction by sequestration of [[SinR]] by [[SinI]] or [[SlrA]] {{PubMed|15661000,19788541}}
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| ** the ''[[epsA]]-[[epsB]]-[[epsC]]-[[epsD]]-[[epsE]]-[[epsF]]-[[epsG]]-[[epsH]]-[[epsI]]-[[epsJ]]-[[epsK]]-[[epsL]]-[[epsM]]-[[epsN]]-[[epsO]]'' operon is not expressed in a ''[[ymdB]]'' mutant {{PubMed|21856853}}
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| ** the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] (this is likely due to the increased stability of the ''[[sinR]]'' mRNA) {{PubMed|21815947}}
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| ** the [[EAR riboswitch]] (eps-associated [[RNA switch]]) located between'' [[epsB]]'' and ''[[epsC]]'' mediates processive antitermination and allows expression of the long eps operon {{PubMed|20374491}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Richard Losick]], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>20735481 </pubmed>
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| ==Original publications==
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| ===The EAR [[RNA switch]]===
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| <pubmed>20374491 20230605 </pubmed>
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| ===Other original publications===
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| <pubmed>15661000,16430695,18047568,18647168 20817675 21856853 21815947 23646920</pubmed>
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| [[Category:Protein-coding genes]]
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