EpsH

• Description: extracellular polysaccharide synthesis
  
  

Gene name	epsH
Synonyms	yveR
Essential	no
Product	unknown
Function	biofilm formation
Gene expression levels in SubtiExpress: epsH
Regulation of this protein in SubtiPathways: epsH
MW, pI	39 kDa, 5.788
Gene length, protein length	1032 bp, 344 aa
Immediate neighbours	epsI, epsG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, EAR riboswitch, RemA regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU34300

Phenotypes of a mutant

• altered cell death pattern in colonies PubMed

Database entries

• BsubCyc: BSU34300

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: glycosyltransferase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU34300

• Structure:

• UniProt: P71057

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed

• Expression browser: epsH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by SinR PubMed

• Regulatory mechanism:
  • SinR: transcription anti-activation (prevents binding of RemA) PubMed
  • RemA: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed
  • the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed
  • the EAR riboswitch (eps-associated RNA switch) located between epsB and epsC mediates processive antitermination and allows expression of the long eps operon PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

<pubmed>20735481 </pubmed>

Original publications

The EAR RNA switch

<pubmed>20374491 20230605 </pubmed>

Other original publications

<pubmed>23012477 15661000,16430695,18047568,11572999 ,15083514 18647168 20817675 21856853 21815947 23646920</pubmed>