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| * '''Description:''' [[two-component systems|two-component]] sensor kinase, control of cellular responses to protein secretion stress <br/><br/>
| | {{#bank: CssS}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cssS''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvqB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] sensor kinase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of cellular responses to protein secretion stress
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33020 cssS]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CssS CssS]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=cssS cssS]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 51 kDa, 5.795
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1353 bp, 451 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cssR]]'', ''[[yirB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33020 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33020 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33020 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:cssS_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cssS_3386398_3387753_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cssS_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33020]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[heat shock proteins]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CssR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU33020
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cssRS.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14132]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of [[CssR]]
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''[[YkoH]], [[KinE]], [[YclK]], [[YvrG]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
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| * '''Modification:''' autophosphorylation on a His residue
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** the extracellular loop domain is required for signal perception {{PubMed|22307758}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[CssS]] is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of [[SubtInteract|interactions]] {{PubMed|21630458}}
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| ** [[CssS]]-[[CssR]]
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| * '''[[Localization]]:'''
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| ** cell membrane (according to Swiss-Prot)
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| ** localized primarily at the division septum but also found in a punctate pattern with lower intensity throughout the cell cylinder {{PubMed|22307758}}
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O32193 O32193]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33020]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[cssR]]-[[cssS]]'' {{PubMed|11555295}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cssS_3386398_3387753_1 cssS] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|12270824}}
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| * '''Regulation:'''
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| ** expressed under conditions of secretion stress ([[CssR]]) {{PubMed|12270824}}
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| * '''Regulatory mechanism:'''
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| ** [[CssR]]: transcription activation {{PubMed|12270824}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>10094672,17600057,21630458,17088376,12270824,11555295, 19820159 21602801 21624103 22307758</pubmed>
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| [[Category:Protein-coding genes]]
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