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| * '''Description:''' allantoate amidohydrolase <br/><br/>
| | {{#bank: PucF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pucF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yurH''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || allantoate amidohydrolase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32530 pucF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pucF pucF]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 5.364
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1236 bp, 412 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucG]]'', ''[[yurI]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32530 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32530 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32530 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yurH_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pucF_3342433_3343671_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pucF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32530]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of nucleotides]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[PucR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU32530
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yurHG.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13994]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' peptidase M20 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2IMO 2IMO] (from ''Escherichia coli k12'', 47% identity, 63% similarity) {{PubMed|17362992}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O32149 O32149]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU32530]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[pucF]]-[[pucG]]'' {{PubMed|12029039}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pucF_3342433_3343671_-1 pucF] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|12029039}}
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| * '''Regulation:'''
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| ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}}
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| * '''Regulatory mechanism:'''
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| ** [[PucR]]: transcription activation {{PubMed|12029039}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>11344136,,12029039,12029039, </pubmed>
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| [[Category:Protein-coding genes]]
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