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| * '''Description:''' Na+/malate symporter <br/><br/>
| | {{#bank: MaeN}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''maeN''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yufR ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || Na+/malate symporter
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| |style="background:#ABCDEF;" align="center"|'''Function''' || malate uptake
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31580 maeN]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=maeN maeN]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 8.411
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1344 bp, 448 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nupQ]]'', ''[[yufS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31580 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31580 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31580 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:maeN_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=maeN_3244770_3246116_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:maeN_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31580]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transporters/ other]]}},
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| {{SubtiWiki category|[[utilization of specific carbon sources]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[MalR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU31580
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12353]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family
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| * '''Paralogous protein(s):''' [[CimH]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O05256 O05256]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31580]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[maeN]]'' {{PubMed|21815947}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=maeN_3244770_3246116_1 maeN] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:''' induced by malate ([[MalR]])
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| * '''Regulatory mechanism:''' [[MalR]]: transcription activation
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| * '''Additional information:'''
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| ** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A642 (''maeN''::Tn917, erm) (available from the [http://www.bgsc.org/ BGSC] or in [[Jörg Stülke]]'s lab)
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| ** 1A642 ( ''maeN''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A642&Search=1A642 BGSC]
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| ** GP1449 (''erm''), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| ** expression of native ''maeN'' in ''B. subtilis'': pGP1951 (in [[pBQ200]]), available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>16339740 </pubmed>
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| ==Original publications==
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| <pubmed>19087206,12949159,,12949159, 21815947</pubmed>
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| [[Category:Protein-coding genes]]
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