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| * '''Description:''' outer spore coat protein, spore associated transglutaminase <br/><br/>
| | {{#bank: Tgl}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''tgl''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yuxF, yugV ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || spore associated transglutaminase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || introduction of crosslinks in the spore coat protein [[GerQ]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31270 tgl]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 6.687
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 735 bp, 245 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpB]]'', ''[[yuzH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31270 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:tgl_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tgl_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31270]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[sporulation proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[GerE regulon]]}},
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| {{SubtiWiki regulon|[[SigE regulon]]}},
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| {{SubtiWiki regulon|[[SigK regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU31270
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tgl.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10946]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH<sub>3</sub> (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as [[GerQ]] (together with [[YabG]]) {{PubMed|16751597}}
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| * '''Protein family:''' bacillus TGase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** outer spore coat, localization depends on [[CotE]] {{PubMed|22171814}}
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P40746 P40746]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31270]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.2.13 2.3.2.13]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[tgl]]'' {{PubMed|9274030}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 tgl] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigK]] {{PubMed|15699190,12501297}}, [[SigE]] {{PubMed|15383836}}
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| * '''Regulation:'''
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| ** expressed late during sporulation in the mother cell ([[SigE]], [[SigK]], [[GerE]]) {{PubMed|15383836,15699190,12501297}}
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| * '''Regulatory mechanism:'''
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| ** [[GerE]]: transcription repression {{PubMed|15383836}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1S122 ( ''tgl''::''erm''), {{PubMed|15317760}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S122&Search=1S122 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Adriano Henriques]], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19797877 </pubmed>
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| ==Original Publications==
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| <pubmed>18249137, 22171814,12501297,16267299,11193401, 16751597 9274030 15699190 15383836 </pubmed>
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| [[Category:Protein-coding genes]]
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