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| * '''Description:''' choline dehydrogenase (FAD-dependent), glycine betaine synthesis <br/><br/>
| | {{#bank: GbsB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gbsB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || choline dehydrogenase (FAD-dependent)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || osmoprotection
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31050 gbsB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gbsB gbsB]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 4.912
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1209 bp, 403 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuaD]]'', ''[[gbsA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31050 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:gbsB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gbsB_3183867_3185075_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gbsB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31050]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[miscellaneous metabolic pathways]]}},
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| {{SubtiWiki category|[[coping with hyper-osmotic stress]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[GbsR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU31050
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11941]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' An alcohol + NAD<sup>+</sup> = an aldehyde or ketone + NADH (according to Swiss-Prot)
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| * '''Protein family:''' iron-containing alcohol dehydrogenase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P71017 P71017]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31050]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.1 1.1.1.1]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' '' [[gbsA]]-[[gbsB]]'' {{PubMed|8752328}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gbsB_3183867_3185075_-1 gbsB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8752328}}
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| * '''Regulation:'''
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| ** induced by choline ([[GbsR]]) {{PubMed|22408163,8752328}}
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| * '''Regulatory mechanism:'''
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| ** [[GbsR]]: transcription repression {{PubMed|22408163}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A1056 ( ''gbsB''::''kan''), {{PubMed|21296969}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1056&Search=1A1056 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Erhard Bremer]], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>,8752328, 21296969 22408163</pubmed>
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| [[Category:Protein-coding genes]]
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