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| * '''Description:''' cystine [[ABC transporter]] (binding protein) <br/><br/>
| | {{#bank: TcyJ}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''tcyJ''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytmJ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || cystine [[ABC transporter]] (binding protein)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || cystine uptake
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29380 tcyJ]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=TcyJ TcyJ]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=tcyJ tcyJ]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 9.602
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 807 bp, 269 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tcyK]]'', ''[[snaA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29380 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ytmJ_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tcyJ_3007426_3008235_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tcyJ_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29380]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ABC transporters]]}},
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| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CymR regulon]]}},
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| {{SubtiWiki regulon|[[AscR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU29380
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ytmIJKLMNO-ytnIJ-ribR-ytnLM.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13884]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' bacterial solute-binding protein 3 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[TcyN]]-([[TcyL]]-[[TcyM]])-([[TcyJ]]-[[TcyK]]) {{PubMed|10092453}}
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| * '''[[Localization]]:'''
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| ** attached to the membrane via [[TcyL]]-[[TcyM]] {{PubMed|10092453}}
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34406 O34406]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29380]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[snaA]]-[[tcyJ]]-[[tcyK]]-[[tcyL]]-[[tcyM]]-[[tcyN]]-[[cmoO]]-[[cmoI]]-[[cmoJ]]-[[ribR]]-[[sndA]]-[[ytnM]]'' {{PubMed|15668000}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tcyJ_3007426_3008235_-1 tcyJ] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15272571}}
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| * '''Regulation:'''
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| ** repressed in the presence of cysteine ([[CymR]]) {{PubMed|16109943}}
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| ** induced in the presence of S-methyl-cysteine ([[AscR]]) {{PubMed|23944997}}
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| * '''Regulatory mechanism:'''
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| ** [[CymR]]: transcription repression {{PubMed|16109943}}
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| ** [[AscR]]: transcription activation {{PubMed|16109943}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A948 ( ''tcyJ''::''kan''), {{PubMed|15262924}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A948&Search=1A948 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>15262924,10092453,16109943,12193636, 15668000 15272571 23944997</pubmed>
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| [[Category:Protein-coding genes]]
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